Motility and fertilizing capacity of fresh and frozen‐thawed spermatozoa in sturgeons Acipenser Baeri and A. ruthenus
Identifieur interne : 000224 ( France/Analysis ); précédent : 000223; suivant : 000225Motility and fertilizing capacity of fresh and frozen‐thawed spermatozoa in sturgeons Acipenser Baeri and A. ruthenus
Auteurs : L. I. Tsvetkova [Russie] ; J. Cosson [France] ; O. Linhart [République tchèque] ; R. Billard [France]Source :
- Journal of Applied Ichthyology [ 0175-8659 ] ; 1996-07.
Abstract
The Siberian sturgeon (Acipenser baeri) and the sterlet (A. ruthenus) were injected with dried sturgeon pituitary (2 mg kg−1), yielding 24 h later respectively 1.71 ± 0.5 and 1.65 ± 0.5 1011 spermatozoa kg−1 body weight. Spermatozoa were best activated with a solution of Tris HC1 50 mM, pH 8.0. The percentage of activated cells was 88 ± 4.4 in A. baeri (n = 5) and 68 ± 19 in A. ruthenus. (n = 5). In A. baeri, immediately after activation, the beat frequency of the flagellum and the mean velocity were in the range of 48–52 Hz and 100–300 μm−1s, respectively. The beat frequency declined to 15 Hz at 30–40 and velocity to 100 μm s−1 at 60 s post‐activation. Only a small percentage of the spermatozoa remained motile after 3–4 min. In all cases spermatozoa showed mostly quasi‐linear trajectories. Sperm was frozen in liquid nitrogen vapor immediately after dilution 1 v: 1 v in a cryopreservation medium (23.4 mM saccharose, 118 mM Tris‐HCl pH 8.0, 20% egg yolk to which 15% DMSO were added). After fast‐thawing procedure (25 s at 40°C), the percentage of motile spermatozoa (once activated in 118 mM Tris‐HCl, pH 8.0) decreased to 23 ± 8.8 in A. baeri and to 15 ± 11 in A. ruthenus. The fertilizing capacity also decreased: 53 ± 8.3% vs. 89 ± 7.6% in control (P < 0.05) in A. baeri and 23 ± 11% vs 53 ± 8.3% in control (P < 0.05) in A. ruthenus. The motility pattern of the surviving frozen/thawed spermatozoa was the same as in fresh spermatozoa.
Url:
DOI: 10.1111/j.1439-0426.1996.tb00071.x
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ISTEX:99475901D7F3FD2996E8E14123544681D1E6CBE6Le document en format XML
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Billard</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:99475901D7F3FD2996E8E14123544681D1E6CBE6</idno><date when="1996" year="1996">1996</date><idno type="doi">10.1111/j.1439-0426.1996.tb00071.x</idno><idno type="url">https://api.istex.fr/document/99475901D7F3FD2996E8E14123544681D1E6CBE6/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001004</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001004</idno><idno type="wicri:Area/Istex/Curation">001002</idno><idno type="wicri:Area/Istex/Checkpoint">000F51</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000F51</idno><idno type="wicri:doubleKey">0175-8659:1996:Tsvetkova L:motility:and:fertilizing</idno><idno type="wicri:Area/Main/Merge">001A63</idno><idno type="wicri:Area/Main/Curation">001956</idno><idno type="wicri:Area/Main/Exploration">001956</idno><idno type="wicri:Area/France/Extraction">000224</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Motility and fertilizing capacity of fresh and frozen‐thawed spermatozoa in sturgeons Acipenser Baeri and A. ruthenus</title><author><name sortKey="Tsvetkova, L I" sort="Tsvetkova, L I" uniqKey="Tsvetkova L" first="L. I." last="Tsvetkova">L. I. Tsvetkova</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>Laboratory of Cryobiology, All‐Russian Institute of Pond Fisheries, Rybnoye, Dmitrov Region, Moscow Area</wicri:regionArea><wicri:noRegion>Moscow Area</wicri:noRegion></affiliation></author><author><name sortKey="Cosson, J" sort="Cosson, J" uniqKey="Cosson J" first="J." last="Cosson">J. Cosson</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>CNRS URA 671, Station Marine, Villefranche sur Mer</wicri:regionArea><wicri:noRegion>Villefranche sur Mer</wicri:noRegion><wicri:noRegion>Villefranche sur Mer</wicri:noRegion></affiliation></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Culture and Hydrobiology, University of South Bohemia, Department of Fish Genetics and Breeding, Vodnany</wicri:regionArea><wicri:noRegion>Vodnany</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">République tchèque</country><wicri:regionArea>Authors' address: O. Linhart, Research Institute of Fish Hydrobiology, University of South Bohemia, Department of Fish Genetics and Breeding, 38925 Vodnany</wicri:regionArea><wicri:noRegion>38925 Vodnany</wicri:noRegion></affiliation></author><author><name sortKey="Billard, R" sort="Billard, R" uniqKey="Billard R" first="R." last="Billard">R. Billard</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire d'Ichtyologie, Museum National d'Histoire Naturelle, Paris Cedex</wicri:regionArea><wicri:noRegion>Paris Cedex</wicri:noRegion><wicri:noRegion>Paris Cedex</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Applied Ichthyology</title><idno type="ISSN">0175-8659</idno><idno type="eISSN">1439-0426</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1996-07">1996-07</date><biblScope unit="volume">12</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="107">107</biblScope><biblScope unit="page" to="112">112</biblScope></imprint><idno type="ISSN">0175-8659</idno></series><idno type="istex">99475901D7F3FD2996E8E14123544681D1E6CBE6</idno><idno type="DOI">10.1111/j.1439-0426.1996.tb00071.x</idno><idno type="ArticleID">JAI107</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0175-8659</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Siberian sturgeon (Acipenser baeri) and the sterlet (A. ruthenus) were injected with dried sturgeon pituitary (2 mg kg−1), yielding 24 h later respectively 1.71 ± 0.5 and 1.65 ± 0.5 1011 spermatozoa kg−1 body weight. Spermatozoa were best activated with a solution of Tris HC1 50 mM, pH 8.0. The percentage of activated cells was 88 ± 4.4 in A. baeri (n = 5) and 68 ± 19 in A. ruthenus. (n = 5). In A. baeri, immediately after activation, the beat frequency of the flagellum and the mean velocity were in the range of 48–52 Hz and 100–300 μm−1s, respectively. The beat frequency declined to 15 Hz at 30–40 and velocity to 100 μm s−1 at 60 s post‐activation. Only a small percentage of the spermatozoa remained motile after 3–4 min. In all cases spermatozoa showed mostly quasi‐linear trajectories. Sperm was frozen in liquid nitrogen vapor immediately after dilution 1 v: 1 v in a cryopreservation medium (23.4 mM saccharose, 118 mM Tris‐HCl pH 8.0, 20% egg yolk to which 15% DMSO were added). After fast‐thawing procedure (25 s at 40°C), the percentage of motile spermatozoa (once activated in 118 mM Tris‐HCl, pH 8.0) decreased to 23 ± 8.8 in A. baeri and to 15 ± 11 in A. ruthenus. The fertilizing capacity also decreased: 53 ± 8.3% vs. 89 ± 7.6% in control (P < 0.05) in A. baeri and 23 ± 11% vs 53 ± 8.3% in control (P < 0.05) in A. ruthenus. The motility pattern of the surviving frozen/thawed spermatozoa was the same as in fresh spermatozoa.</div></front></TEI><affiliations><list><country><li>France</li><li>Russie</li><li>République tchèque</li></country></list><tree><country name="Russie"><noRegion><name sortKey="Tsvetkova, L I" sort="Tsvetkova, L I" uniqKey="Tsvetkova L" first="L. I." last="Tsvetkova">L. I. Tsvetkova</name></noRegion></country><country name="France"><noRegion><name sortKey="Cosson, J" sort="Cosson, J" uniqKey="Cosson J" first="J." last="Cosson">J. Cosson</name></noRegion><name sortKey="Billard, R" sort="Billard, R" uniqKey="Billard R" first="R." last="Billard">R. Billard</name></country><country name="République tchèque"><noRegion><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name></noRegion><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O." last="Linhart">O. Linhart</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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